Antiviral activity of garlic extract on Influenza virus

نویسندگان

  • E Amini Pasteur Institute of IRAN (Influenza Unit)
  • P Mehrbod Pasteur Institute of IRAN (Influenza Unit)
چکیده مقاله:

Abstract: Influenza virus is the most important cause of annual morbidities and mortalities worldwide with numerous antigenic drifts and shifts. Inaccessibility to effective drugs and vaccines has made world health authorities to be interested in traditional medicine in order to prevent spread of the infectious agent. Garlic is one of the most famous of all plants in human history. It has been shown that garlic extract has various effects on different diseases.  The aim of this study was to evaluate garlic extract antiviral activity against influenza virus in cell culture. To study the potential antiviral activity, MDCK (Madin-Darbey Canin Kidney) cells were treated with effective minimal cytotoxic concentration of the extract and 100 TCID50 (50% Tissue Culture Infectious Dose) of the virus during infection at different time periods. The viral titers were determined by hemagglutination (HA) and TCID50 assays. The antiviral effect of the extract was studied at 1, 8 and 24 hours after treatment on the culture. To measure the amount of the viral genome synthesized at different times after treatment, RNA extraction, Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and free band densitometry software were performed. Although the precise mechanism has not been defined yet, it was found that garlic extract with a good selectivity index (SI) has inhibitory effect on the virus penetration and proliferation in cell culture.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

antiviral activity of garlic extract on influenza virus

background and aims: influenza virus is the most important cause of annual morbidities and mortalities worldwide with numerous antigenic drifts and shifts. inaccessibility to effective drugs and vaccines has made world health authorities to be interested in traditional medicine in order to prevent spread of the infectious agent. garlic is one of the most famous of all plants in human history. i...

متن کامل

the effect of aqueous extract of garlic on formalin-induced pain in male rats

چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...

15 صفحه اول

Antiviral Activity of Arnebia Euchroma and Amniotic Membrane against HSV-1, Rotavirus, and Influenza Virus

Background and Aims: Currently, there are antiviral chemicals used to treat viral infections accompanied by limitations such as high levels of toxicity and adverse effects in humans, the emergence of drug-resistant viral strains, low numbers, and limited diversity. Therefore, it is necessary to evaluate new photochemical to obtain new therapeutic methods. The present study was conducted to eval...

متن کامل

Antimicrobial Activity of Garlic Extract Against Shigella

Some beliefs suggest that garlic is an excellent natural antimicrobial drug that can be considered as an alternative form of treatment or prophylaxis in gastrointestinal infections. The purpose of this study is to determine quantitative antimicrobial activity of garlic against Shigella by detecting the MIC. Garlic extracts were obtained and dilution susceptibility assay was used to determine th...

متن کامل

antioxidant and antimicrobial activity of allium jesdianum extract

چکیده بررسی فعالیت ضد اکسیدانی و ضدمیکروبی عصاره گیاه بن سرخ (allium jesdianum) به کوشش: زهرا مقیمی هدف از این تحقیق بررسی میزان ترکیبات فنولی کل، ترکیبات فلاوونوئیدی کل، فعالیت ضداکسیدانی و ضدمیکروبی عصاره ی گیاه تازه و خشک بن سرخ بود. استخراج عصاره به روش های غوطه وری، استفاده از همزن مغناطیسی، دستگاه مایکروویو، امواج فراصوت و گرمادهی مقاومتی و با استفاده از متانول، اتانول و آب به عنوا...

Antiviral activity of ginseng extract against respiratory syncytial virus infection

Panax ginseng has been known to have a number of immuno-modulatory effects. In this study, we investigated whether Panax Korean red ginseng extract (KRGE) has in vitro and in vivo antiviral effects on respiratory syncytial virus (RSV) infection. KRGE improved the survival of human lung epithelial cells against RSV infection and inhibited RSV replication. In addition, KRGE treatment suppressed t...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 3  شماره None

صفحات  19- 23

تاریخ انتشار 2009-06

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023